Human CD276/B7-H3 (Leu29-Pro245) protein, with C-mFc and 6xHis Tag, expressed from HEK293

Home/ Products / ECD and Other Proteins

PRODUCT

  • SKU

    DMP1252-50ug

  • Size

    50 ug

  • Price

    $560

1
  • SKU

    DMP1252-100ug

  • Size

    100 ug

  • Price

    $840

1
This recombinant protein contains Leu29-Pro245 of Uniprot ID Q5ZPR3. It has a C-mFc and 6xHis Tag, with a calculated molecular weight of 70-75 kDa.
gene name CD276
gene id Uniprot ID: Q5ZPR3
alias B7-H3, CD276, B7 homolog 3
host species Human
production host HEK293
tags C-mFc and 6xHis Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 2 ug/mL (100 uL/well) Human B7-H3 Protein, mFc-His Tag binds to anti-B7-H3 (TAA06 biosimilar) mAb in a linear range of 0.64?16 ng/mL.

Human B7-H3 Protein, mFc-His Tag, SDS-PAGE analysis under reducing conditions.

ELISA plate coated with 2 ug/ml (100 ul/well) Human B7-H3, mFc-His tagged protein binds to anti-B7-H3 neutralizing antibody ([getskuurl sku"in a linear range of 0.24-31.25 ng/ml.


PRODUCT

  • SKU

    DMP1252-50ug

  • Size

    50 ug

  • Price

    $560

1
  • SKU

    DMP1252-100ug

  • Size

    100 ug

  • Price

    $840

1