Human CD40LG/CD40 Ligand (Met113-Leu261) protein, with N-hFc Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP1281-50ug

  • Size

    50 ug

  • Price

    $543

1
  • SKU

    DMP1281-100ug

  • Size

    100 ug

  • Price

    $805

1
This recombinant protein contains Met113-Leu261 of Uniprot ID P29965. It has a N-hFc Tag, with a calculated molecular weight of 42.3 kDa. In SDS-PAGE, the protein migrates at 40-53 kDa due to glycosylation.
gene name CD40LG
gene id Uniprot ID: P29965
alias CD40LG, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39
host species Human
production host HEK293
tags N-hFc Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

Human CD40 Ligand Protein, hFc Tag, SDS-PAGE analysis under reducing conditions.

ELISA plate coated with 2 ug/ml (100 ul/well) Human CD40L, hFc tagged protein binds to anti-CD40L neutralizing antibody in a linear range of 0.64-400 ng/ml.

ELISA plate coated with 2 ug/ml (100 ul/well) Human CD40 Ligand,hFc tagged protein binds to Human CD40, mFc-His tagged protein in a linear range of 0.51-320 ng/ml.


PRODUCT

  • SKU

    DMP1281-50ug

  • Size

    50 ug

  • Price

    $543

1
  • SKU

    DMP1281-100ug

  • Size

    100 ug

  • Price

    $805

1