Human/Mouse FGF8/FGF-8B (Gln23-Arg215) protein, tag-free, expressed from E.coli

PRODUCT

This recombinant protein contains Gln23-Arg215 of Uniprot ID P55075-3. It is tag-free, with a molecular weight of 22.5 KDa.

gene name	FGF8
gene id	Uniprot ID: P55075-3
alias	Fibroblast growth factor 8, Androgen-induced growth factor, Heparin-binding growth factor 8, AIGF, HBGF-8, FGF-8B
host species	Human/Mouse
production host	E.coli
tags	None
purity	Greater than 95% as determined by reducing SDS-PAGE.

endotoxin	No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation	Lyophilized from a 0.2 um filtered solution of PBS, pH 7.4.
reconstitution	Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping	Shipped at ambient conditions.
storage	Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 21.87 ng/ml.

Purity greater than 95% as determined by reducing SDS-PAGE.