Human ICOSLG/B7-H2 (Asp19-Thr256) protein, with C-mFc and 6xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP1655-50ug

  • Size

    50 ug

  • Price

    $578

1
  • SKU

    DMP1655-100ug

  • Size

    100 ug

  • Price

    $875

1
This recombinant protein contains Asp19-Thr256 of Uniprot ID O75144. It has a C-mFc and 6xHis Tag, with a calculated molecular weight of 53.9 kDa.
gene name ICOSLG
gene id Uniprot ID: O75144
alias ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS, ICOS ligand
host species Human
production host HEK293
tags C-mFc and 6xHis Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 1 ug/mL (100 uL/well) Human B7-H2 Protein, mFc-His Tag binds to anti-B7-H2 Rabbit mAb in a linear range of 3.2-80 ng/mL.

HEK293 cell line transfected with irrelevant protein (B) and human ICOS (A) were surface stained with Human B7-H2, mFc-His tagged protein 1ug/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Human B7H2 Protein, mFc-His Tag, SDS-PAGE analysis under reducing conditions.


PRODUCT

  • SKU

    DMP1655-50ug

  • Size

    50 ug

  • Price

    $578

1
  • SKU

    DMP1655-100ug

  • Size

    100 ug

  • Price

    $875

1