Human IL7/IL-7 protein, tag-free, expressed from E.coli

Home/ Products / ECD and Other Proteins

PRODUCT

  • SKU

    DMP1782-50ug

  • Size

    50 ug

  • Price

    $880

1
This recombinant protein contains the amino acid sequence of Uniprot ID P13232. It is tag-free, with a molecular weight of 17.5 KDa.
gene name IL7
gene id Uniprot ID: P13232
alias Interleukin-7, IL-7, IL7
host species Human
production host E.coli
tags None
purity Greater than 95% as determined by reducing SDS-PAGE.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from a 0.2 um filtered solution of 20mM PB, 300mM NaCl, pH 8.0.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.02-0.08 ng/ml.

Loaded Human IL-7RA-His on HIS1K Biosensor, binds to Human IL-7 with an affinity constant of 15.04 nM as determined in BLI assay.

Loaded Human IL-7RA-Fc-His on HIS1K Biosensor, binds to Human IL-7 with an affinity constant of 18.59 nM as determined in BLI assay.

Loaded Mouse IL-7RA-Fc on Protein A Biosensor, binds to Human IL-7 with an affinity constant of 29.2 nM as determined in BLI assay.

Purity greater than 95% as determined by reducing SDS-PAGE.


PRODUCT

  • SKU

    DMP1782-50ug

  • Size

    50 ug

  • Price

    $880

1