Human MICA (Glu24-Trp307) protein, with C-6xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP1884-50ug

  • Size

    50 ug

  • Price

    $578

1
  • SKU

    DMP1884-100ug

  • Size

    100 ug

  • Price

    $875

1
This recombinant protein contains Glu24-Trp307 of Uniprot ID Q29983. It has a C-6xHis Tag, with a calculated molecular weight of 33.5 kDa. In SDS-PAGE, the protein migrates at 55-70 kDa due to glycosylation.
gene name MICA
gene id Uniprot ID: Q29983
alias MIC-A, PERB11.1
host species Human
production host HEK293
tags C-6xHis Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 2 ug/ml (100 ul/well) Human MICA, His tagged protein binds to Human NKG2D, mFc tagged protein in a linear range of 0.064-1.6 ug/ml.

Flow cytometry analysis of 15 ug/mL Human MICA Protein, His Tag on Expi293 cells transfected with Human NKG2D protein and Human DAP10 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Human MICA Protein, His Tag, SDS-PAGE analysis under reducing conditions.


PRODUCT

  • SKU

    DMP1884-50ug

  • Size

    50 ug

  • Price

    $578

1
  • SKU

    DMP1884-100ug

  • Size

    100 ug

  • Price

    $875

1