Human NCR3/NKp30 (Leu19-Glu135) protein, with C-hFc Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP1928-50ug

  • Size

    50 ug

  • Price

    $578

1
  • SKU

    DMP1928-100ug

  • Size

    100 ug

  • Price

    $875

1
This recombinant protein contains Leu19-Glu135 of Uniprot ID O14931. It has a C-hFc Tag, with a calculated molecular weight of 39.0 kDa. In SDS-PAGE, the protein migrates at 45-60 kDa due to glycosylation.
gene name NCR3
gene id Uniprot ID: O14931
alias NCR3, CD337, NKp30, 1C7, LY117, MALS
host species Human
production host HEK293
tags C-hFc Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 2 ug/ml (100 ul/well) Human B7H6, His tagged protein binds to Human NKp30, hFc tagged protein in a linear range of 250-2000 ng/ml.

B7H6 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis of 2ug/ml Human NKp30 Protein, hFc Tag on Expi293 cells transfected with human B7H6 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Human NKp30 Protein, hFc Tag, SDS-PAGE analysis under reducing conditions.


PRODUCT

  • SKU

    DMP1928-50ug

  • Size

    50 ug

  • Price

    $578

1
  • SKU

    DMP1928-100ug

  • Size

    100 ug

  • Price

    $875

1