Human SIRPA (Glu31-Tyr373) protein, with C-mFc Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP2096-50ug

  • Size

    50 ug

  • Price

    $543

1
  • SKU

    DMP2096-100ug

  • Size

    100 ug

  • Price

    $805

1
This recombinant protein contains Glu31-Tyr373 of Uniprot ID P78324. It has a C-mFc Tag, with a calculated molecular weight of 63.8 kDa. In SDS-PAGE, the protein migrates at 70-100 kDa due to glycosylation.
gene name SIRPA
gene id Uniprot ID: P78324
alias SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS
host species Human
production host HEK293
tags C-mFc Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 1 ug/ml (100 ul/well) Human SIRP&alpha; Protein, mFc Tag binds to anti-SIRP&alpha; antibody, Rabbit mAb clone: DM8 in a linear range of 3.2-80 ng/mL.

Human SIRP&alpha; Protein, mFc Tag, SDS-PAGE analysis under reducing conditions.

ELISA plate coated with 2 ug/mL (100 uL/well) Human SIRP&alpha; Protein, mFc Tag binds to Human CD47 full length protein-synthetic nanodisc in a linear range of 80?2000 ng/mL.


PRODUCT

  • SKU

    DMP2096-50ug

  • Size

    50 ug

  • Price

    $543

1
  • SKU

    DMP2096-100ug

  • Size

    100 ug

  • Price

    $805

1