Human TNFRSF8/CD30 (Phe19-Lys379) protein, with C-6xHis Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP2196-50ug

  • Size

    50 ug

  • Price

    $648

1
  • SKU

    DMP2196-100ug

  • Size

    100 ug

  • Price

    $971

1
This recombinant protein contains Phe19-Lys379 of Uniprot ID P28908. It has a C-6xHis Tag, with a calculated molecular weight of 39.3 kDa. In SDS-PAGE, the protein migrates at 55-70 kDa due to glycosylation.
gene name TNFRSF8
gene id Uniprot ID: P28908
alias TNFRSF8, CD30, D1S166E, Ki-1
host species Human
production host HEK293
tags C-6xHis Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

Human CD30 Protein, His Tag, SDS-PAGE analysis under reducing conditions.

ELISA plate coated with 2 ug/ml (100 ul/well) Human CD30, His tagged protein binds to anti-CD30 neutralizing antibody in a linear range of 0.13-80.0 ng/ml.

ELISA plate coated with 2 ug/ml (100 ul/well) Human CD30, His tagged protein binds to Human CD30 Ligand,mFc-His tagged protein in a linear range of 2.56-320 ng/ml.

Flow cytometry analysis of 1 ug/mL Human CD30 Protein, His tag on Expi293 cells transfected with human CD30L (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).


PRODUCT

  • SKU

    DMP2196-50ug

  • Size

    50 ug

  • Price

    $648

1
  • SKU

    DMP2196-100ug

  • Size

    100 ug

  • Price

    $971

1