Human TNFSF14/LIGHT (Leu83-Val240) protein, with N-hFc Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP2207-50ug

  • Size

    50 ug

  • Price

    $578

1
  • SKU

    DMP2207-100ug

  • Size

    100 ug

  • Price

    $875

1
This recombinant protein contains Leu83-Val240 of Uniprot ID O43557. It has a N-hFc Tag, with a calculated molecular weight of 43.4 kDa.
gene name TNFSF14
gene id Uniprot ID: O43557
alias TNFSF14, CD258, HVEML, LIGHT, LTg
host species Human
production host HEK293
tags N-hFc Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 2 ug/ml (100 ul/well) Human HVEM, His tagged protein binds to Human LIGHT, hFc tagged protein in a linear range of 0.32-40 ng/ml.

Flow cytometry analysis of 15 ug/mL Human LIGHT Protein, hFc tag on Expi293 cells transfected with human HVEM (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Human LIGHT Protein, hFc Tag, SDS-PAGE analysis under reducing conditions.


PRODUCT

  • SKU

    DMP2207-50ug

  • Size

    50 ug

  • Price

    $578

1
  • SKU

    DMP2207-100ug

  • Size

    100 ug

  • Price

    $875

1