Mouse anti-DYKDDDDK (same to FLAG tag) monoclonal antibody

Mouse anti-DYKDDDDK (same to FLAG tag) monoclonal antibody

SKU Description Size Price Shipping  
LYT1008 Mouse anti-DYKDDDDK (same to FLAG tag) monoclonal antibody 100 µg $245 2-5 Days Add To Shopping Cart
Datasheet
DescriptionThe immunogen of this antibody is a synthetic peptide (DYKDDDDK) coupled to KLH. The antibody recognizes the same FLAG tag/epitope of Sigma-Aldrich, but its affinity is stronger than Sigma's M2 antibody, thus making it superior in applications such as WB, IP, ChIP, etc.
Gene IDN/A
Gene NameFLAG Tag
AliasN/A
Host SpeciesMouse
Reactivity SpeciesFLAG-tagged proteins.
ClonalityMonoclonal
Clone NumberSPD26
IsotypeIgG
ConjugationNone
Concentration1 mg/ml
FormulationPBS pH 7.2, 0.09% NaN3
Purification MethodAntibody was affinity purified with Protein A column and corresponding antigen column in tantem.
ApplicationsWB, IF, IP
Suggested DilutionsWB: 1/5000; IHC: 1/200. Optimal dilutions should be determined experimentally by end users
Storage ConditionsStable for one year at -20°C, or 4°C for 3 months from date of shipment. Aliquot and avoid repeated freezing and thawing for long term storage.
Shipping ConditionsThis product is shipped with blue ice.
Background
Antibody Image
WB analysis of 293 T cells expressing Flag tag (left) and IF analysis of Hela cells expressing Flag tag (right).