Mouse anti-His tag monoclonal antibody

Mouse anti-His tag monoclonal antibody

SKU Description Size Price Shipping  
LYT1012 Mouse anti-His tag monoclonal antibody 100 µg $245 2-5 Days Add To Shopping Cart
Datasheet
DescriptionThe immunogen is a synthetic 6xHis peptide coupled to KLH. The antibody recognizes His fusion proteins.
Gene IDN/A
Gene NameHIS Tag
AliasN/A
Host SpeciesMouse
Reactivity SpeciesHIS-tagged proteins.
ClonalityMonoclonal
Clone NumberSPD1
IsotypeIgG
ConjugationNone
Concentration1 mg/ml
FormulationPBS pH 7.2, 0.09% NaN3
Purification MethodAntibody was affinity purified with Protein A column and corresponding antigen column in tantem.
ApplicationsWB, IF, IP
Suggested DilutionsWB: 1/5000; IHC: 1/200. Optimal dilutions should be determined experimentally by end users
Storage ConditionsStable for one year at -20°C, or 4°C for 3 months from date of shipment. Aliquot and avoid repeated freezing and thawing for long term storage.
Shipping ConditionsThis product is shipped with blue ice.
Background
Antibody Image
Western blot analysis of an His-tag fusion (upper) and IF analysis of a cell line stably expressing an His-tagged protein (lower right panel. Lower Left panel is a negative control)