Anti-mouse CD25(IL-2Ra), FITC, Clone PC-61.5.3

Anti-mouse CD25(IL-2Ra), FITC, Clone PC-61.5.3

SKU Description Size Price Shipping  
ZYF1071 Anti-mouse CD25(IL-2R), FITC, Clone PC-61.5.3 500 µg $213 Immediate Add To Shopping Cart
ZYF1072 Anti-mouse CD25(IL-2R), FITC, Clone PC-61.5.3 50 µg $58 Immediate Add To Shopping Cart
Datasheet
DescriptionAnti-mouse CD25(IL-2Ra) antibody, conjugated with FITC. This antibody has been extensively tested in flow cytometry.
Gene ID16184
Gene NameIl2ra
AliasNot listed
Host SpeciesRat
Reactivity SpeciesMouse
ClonalityMonoclonal
Clone NumberPC-61.5.3
IsotypeIgG1,lambda
ConjugationFITC
Concentration0.5 mg/ml
FormulationPBS pH 7.2, 0.09% NaN3,0.2% BSA
Purification MethodAntibody was purified by affinity chromatography and conjugated with dye. The conjugated antibody was polished with chromatography and is free of unconjugated dye and unconjugated antibody.
ApplicationsFlow Cytometry
Suggested DilutionsSuggested amount of the reagent to be used: <0.5 µg antibody for 106 cells in 100 µl staining buffer. Optimal dilutions must be determined by end users.
Storage ConditionsStore at 4°C and protect from light. DO NOT FREEZE. Stable for at least six months from date of shipment.
Shipping ConditionsAt Ambient Temperature
BackgroundThe interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. This antibody was manufactured by Sungene Biotech USA.
Antibody Image
C57BL/6 mouse splenocytes stained with FITC Anti-mouse CD25 (y-axis) and PE Anti-mouse CD4 (x-axis)