SPEED 2X PCR mix with regular Taq for genotying and clone screening

SPEED 2X PCR mix with regular Taq for genotying and clone screening

SKU Description Size Price Shipping  
LYM1001 SPEED 2X PCR mix with regular Taq 5 mL/200 rxns $138 Immediate Add To Shopping Cart
LYM1002 SPEED 2X PCR mix with regular Taq and Loading Dye 5 mL/200 rxns $138 Immediate Add To Shopping Cart
Datasheet:
The PCR Taq Mastermix is a ready-to-use mixture of high quality Taq DNA Polymerase, deoxynucleotides, and reaction buffer in a 2X concentrate. It contains all the necessary reagents for amplification of DNA. To set up a PCR reaction, just add DNA template, primers and water. Amplification with this mix generates PCR products with 3'-A overhangs.

Storage

Keep at -20°C for long term storage. Stable at 4°C for three months or for fifteen freeze-thaw cycles. It is recommended to keep an aliquot at 4°C for daily use.

Protocol

  1. Add the following components to a sterile tube siting on ice.
    ComponentsVolume
    2X PCR MasterMix25µl
    DNA Template~100ng
    Forward Primer (10 µM)1µl
    Reverse Primer (10 µM)1µl
    H2Oto 50µl
  2. Mix contents of tube and centrifuge briefly.
  3. Incubate tube in a thermal cycler at 94°C for 3 min to completely denature the template.
  4. Perform 30-40 cycles of PCR amplification as follow:
    Denature: 94°C for 30 sec
    Anneal: 55°C for 30 sec (annealing temperature may need to be adjusted based on Tm)
    Extend: 72°C for 1 min/1kb template
  5. Analyze the amplification products.
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