SPEED 2X PCR mix with Taq Plus for Hi-fidelity amplification

SPEED 2X PCR mix with Taq Plus for Hi-fidelity amplification

SKU Description Size Price Shipping  
LYM1003 SPEED 2X PCR mix with Taq Plus for Hi-fidelity amplification 5 mL/200 rxns $162 2-5 Days Add To Shopping Cart
LYM1004 SPEED 2X PCR mix with Taq Plus and Loading Dye 5 mL/200 rxns $162 2-5 Days Add To Shopping Cart
Datasheet:
The PCR Taq Plus Mastermix is a ready-to-use mixture of high quality Taq Plus DNA Polymerase, deoxynucleotides, and reaction buffer in a 2X concentrate. It contains all the necessary reagents for amplification of DNA. To set up a PCR reaction, just add DNA template, primers and water. Amplification with this mix generates PCR products with 3'-A overhangs.

Storage

Keep at -20°C for long term storage. Stable at 4°C for three months or for fifteen freeze-thaw cycles. It is recommended to keep an aliquot at 4°C for daily use.

Protocol

  1. Add the following components to a sterile tube siting on ice.
    ComponentsVolume
    2X PCR MasterMix25µl
    DNA Template~100ng
    Forward Primer (10 µM)1µl
    Reverse Primer (10 µM)1µl
    H2Oto 50µl
  2. Mix contents of tube and centrifuge briefly.
  3. Incubate tube in a thermal cycler at 94°C for 3 min to completely denature the template.
  4. Perform 30-40 cycles of PCR amplification as follow:
    Denature: 94°C for 30 sec
    Anneal: 55°C for 30 sec (annealing temperature may need to be adjusted based on Tm)
    Extend: 72°C for 1 min/1kb template
  5. Analyze the amplification products.
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