SPEED TaqProbe 2X qPCR Mastermix with ROX

SPEED TaqProbe 2X qPCR Mastermix with ROX

SKU Description Size Price Shipping  
LYM1012 SPEED TaqProbe 2X qPCR Mastermix with ROX 4 x 1.25 mL $186 Immediate Add To Shopping Cart
Datasheet:
SPEED TaqProbe 2X qPCR Mastermix is designed for TaqMan probe-based real-time PCR analysis of DNA samples. The components of TaqProbe qPCR Mastermix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. Multiplex formulation supports quantitative amplification and detection of up to four targets simultaneously and shows consistent high performance.

Size

5 ml (1.25ml x 4 tubes)

Usage    

SNP Genotyping assays
Gene expression analysis
Microarray validation
High throughput screening

Components    

TaqProbe qPCR Mastermix is a 2X mix of dNTPs, Hotstart Taq polymerase, MgCl2, reference dye, and proprietary buffer components

Storage    

Keep at -20°C. Once thawed, store at 4°C for up to 3 months.

Reaction Setup

TaqProbe 2X PCR Mix:       10 µl
Template DNA:                 < 10ng
TaqMan Probe:               100-300nM
Forward Primer:              100-500nM
Reverse Primer:              100-500nM
Water:                               to 20µl


Cycling Program

Activation at 95°C for 10 min
Followed by 40 Cycles with:
    Denature:  95°C 15 sec
    Anneal/Extension:   60°C 1  min

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