SPEED ECL Western Blotting Detection Reagents

SPEED ECL Western Blotting Detection Reagents

SKU Description Size Price Shipping  
LYP1021 SPEED ECL Western Blotting Detection Reagents 2 x 100 mL $188 Immediate Add To Shopping Cart
Datasheet:
Components    

Solution A: 100 ml
Solution B: 100 ml

Sensitivity    

Detects picogram amounts of antigen with WB

Storage    

Keep at 4°C in dark. Stable for 1 year

Protocol

1.    Wash membrane thoroughly after antibody incubation and before initiating ECL
2.    Immediately before use, mix solutions A and B in a 1:1 ratio in a low light intensity environment. 10mL of solution is recommended for every 10 cm2 of membrane.
3.    Add the reagents onto membrane and incubate for 1 minute while shaking.
4.    Pour out the solution, dab off any excess solution and wrap the blot in clear plastic
5.    Place the membrane into a film cassette and expose to X-ray film in a dark-room immediately.
6.    Develop the exposed film.

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