BHK-21 (C-13) (Baby Hamster Kidney Cells) whole cell lysate

BHK-21 (C-13) (Baby Hamster Kidney Cells) whole cell lysate

SKU Description Size Price Shipping  
LYP1117 BHK-21 (C-13) (Baby Hamster Kidney Cells) whole cell lysate 100 µg $198 2-5 Days Add To Shopping Cart
Datasheet:
SPEED BIOSYSTEMS provides a variety of ready-to-use cell line lysates for proteomics research. The lysates are perfect for WB, IP, protein-protein interaction analysis and tissue specific expression identification.

We also provide Nuclear Extract from Hela and HEK293 cell lines for intro transcription of mammalian genes.

Preparation

Cell lysates were prepared by homogenizing cells in modified RIPA buffer (150 mM NaCl; 50 mM Tris-HCl pH 7.4; 1 mM EDTA; 1 mM PMSF; 1% Triton X-100; 1% sodium deoxycholic acid; 0.1% SDS, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with BCA assay.

Size/Concentration

100 µg, 1 mg/ml 

Application

Optimal amount should be determined by end users. We recommend using 20 µg of lysates for WB.

Storage

Store at -80°C. Stable for at least 1 year after shipping date.
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