HeLa Cell Nuclear Extract (for in vitro transcription)

HeLa Cell Nuclear Extract (for in vitro transcription)

SKU Description Size Price Shipping  
OP00011 HeLa Cell Nuclear Extract (for in vitro transcription) 200µg $155 Immediate Add To Shopping Cart
OP00012 HeLa Cell Nuclear Extract (for in vitro transcription) 500µg $311 Immediate Add To Shopping Cart
Other VersionsNone
Datasheet
DescriptionThe HeLa cell nuclear extract of this catalog number is specifically recommended for: 1) in vitro transcription; 2) protein-DNA/RNA and protein-protein interactions; and 3) as a source of individual transcription factors. Although this extract also contains most pre-mRNA processing factors, it is, however, not recommended for in vitro pre-mRNA processing.

The HeLa cell nuclear extract was prepared as described by Dignam et al. and Manley et al. . This extract contains all basal transcription factors, including TFIIA, -IIB, -IID, -IIE, -IIF, -IIH and RNA polymerase II, as well as most gene-specific activators and cofactors, such as Sp1, Oct-1, NF-kB, USF, ATF, PC4, p300 etc. Therefore, it has been used as the source of transcription factors for the cell-free transcription system to study specific transcription by RNA polymerase II as well as RNA polymerase III and for purifying transcription factors.

1. Dignam, J.D., et al., (1983) Nucleic Acids Res. 11, 1475-1489
2. Manley, J.L., et al., (1980) Proc. Natl. Acad. Sci. USA 77, 5706-5710
Gene IDNA
Gene NameNA
AliasNA
Host SpeciesHuman
Production HostHela cell
TagsNo Tag
Purity> 95% as determined by SDS-PAGE
Bio Activity1-5 µl is sufficient for a gel mobility shift assay in a 20 µl reaction; 5-10 µl is sufficient for in vitro transcription assay and 20-50 µl is sufficient for a protein-protein interaction assay.
EndotoxinNot Tested
Formulation20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
Concentration4 mg/ml
ReconsitutionAlready in liquid form.
Storage-80 °C
ShippingDry Ice
Background
NA
Protein Image