293 Cell Nuclear Extract (for in vitro pre-mRNA splicing)

293 Cell Nuclear Extract (for in vitro pre-mRNA splicing)

SKU Description Size Price Shipping  
OP00031 293 Cell Nuclear Extract (for in vitro pre-mRNA splicing) 200µg $155 Immediate Add To Shopping Cart
OP00032 293 Cell Nuclear Extract (for in vitro pre-mRNA splicing) 500µg $311 Immediate Add To Shopping Cart
Other VersionsNone
Datasheet
DescriptionThe 293 cell nuclear extract is specifically recommended for 1) in vitro splicing; 2) protein-DNA/RNA and protein-protein interactions; and 3) source of individual splicing factors. Although this extract also contains most transcription factors, it is not recommended for in vitro transcription assays.
The 293 cell nuclear extract was prepared as described . Although this extract contains all basal transcription factors, as well as most gene-specific activators and cofactors, it is prepared for the purpose of pre-mRNA splicing, especially for cell-specific polyadenylation, DNA replication and pre-mRNA splicing. 293 cell nuclear extract has been used as the source of individual splicing factors (such as ASF/SF2), and for the cell free system to study the mechanism of pre-mRNA processing.
1. Noble, J.C.S. et al., (1987) Cell 50, 227-236
2. Krainer, A.R., et al., (1984) Cell 36, 993-1005
3. Ge, H., et al., (1990) Cell 62, 25-34
Gene IDNA
Gene NameNA
AliasNA
Host SpeciesHuman
Production HostHEK293 cell
TagsNo Tag
Purity> 95% as determined by SDS-PAGE
Bio Activity1-5 µl is sufficient for a gel mobility shift assay in a 20 µl reaction; 5-10 µl is sufficient for in vitro splicing assay and 20-50 µl is sufficient for a protein-protein interaction assay.
EndotoxinNot Tested
Formulation20 mM HEPES-Na (pH 7.9), 20% Glycerol, 42 mM (NH4)2SO4, 0.5 mM DTT and 0.2 mM EDTA.
Concentration7.5 mg/ml
ReconsitutionAlready in liquid form.
Storage-80 °C
ShippingDry Ice
Background
NA
Protein Image