HeLa Cell Cytosol Extract (S100)

HeLa Cell Cytosol Extract (S100)

SKU Description Size Price Shipping  
OP00041 HeLa Cell Cytosol Extract (S100) 200µg $155 Immediate Add To Shopping Cart
OP00042 HeLa Cell Cytosol Extract (S100) 500µg $311 Immediate Add To Shopping Cart
Other VersionsNone
Datasheet
DescriptionThe S100 cytoplasmic extract is specifically recommended for 1) in vitro splicing; 2) protein-DNA/RNA and protein-protein interactions; and 3) source of individual splicing factors and other regulatory proteins.
Gene IDNA
Gene NameNA
AliasNA
Host SpeciesHuman
Production HostHela cell
TagsNo Tag
Purity> 95% as determined by SDS-PAGE
Bio Activity1-5 µl is sufficient for a gel mobility shift assay in a 20 µl reaction; 5-10 µl is sufficient for in vitro splicing assay and 20-50 µl is sufficient for a protein-protein interaction assay.
EndotoxinNot Tested
Formulation20 mM HEPES-Na (pH 7.9), 20% Glycerol, 42 mM (NH4)2SO4, 0.5 mM DTT and 0.2 mM EDTA.
Concentration6 mg/ml
ReconsitutionAlready in liquid form.
Storage-80 °C
ShippingDry Ice
Background
The development of cell-free systems that accurately process pre-mRNA by splicing
polyadenylation and editing has led to insights into the mechanisms and the biochemical characteristics of these reactions (1-3). Splicing of pre-mRNA requires the presence of specific sequence elements (cis factors) as well as many protein factors. Although the splicing reaction proceeds in the nucleus
mA ny spL icinG fA cT ors hA ve been founD exisT inG in ceL L cyT opL A sm. MosT essenT iA L spL icinG fA cT ors incL uD inG snRNPs
Protein Image