Human GTF2E1 Protein (TFIIEa p56, Transcription Factor IIE alpha subunit), Made in E.coli, with His Tag

Human GTF2E1 Protein (TFIIEa p56, Transcription Factor IIE alpha subunit), Made in E.coli, with His Tag

SKU Description Size Price Shipping  
OP10051 Human GTF2E1 Protein (TFIIEa p56, Transcription Factor IIE alpha subunit), Made in E.coli, with His Tag 10µg $271 Immediate Add To Shopping Cart
OP10052 Human GTF2E1 Protein (TFIIEa p56, Transcription Factor IIE alpha subunit), Made in E.coli, with His Tag 25µg $541 Immediate Add To Shopping Cart
Other VersionsGTF2E1 with His Tag
GTF2E1 with GST Tag
GTF2E1/GTF2E2 mixture
Biotin-labeled and heavy-labeled (C13 and N15) versions of this protein are also available. Please contact us for more information.
Datasheet
DescriptionThe human Transcription Factor IIE (TFIIE) is composed of 56 kDa (a) and 34 kDa (ß) subunits and is shown to be a heterotetramer . The 56-kDa subunit contains a region similar to a zinc-binding domain and a region sharing homology with the catalytic loop of a kinase domain . TFIIE binds to RNA polymerase II in solution and joins the pre-initiation complex probably concomitant with RNA polymerase II and TFIIF
Gene ID2960
Gene NameGTF2E1
AliasFE, TF2E1
Host SpeciesHuman
Production HostE.coli
TagsHis Tag
Purity> 95% as determined by SDS-PAGE
Bio Activity1 unit equals 1 nanogram of purified protein. 20 units are sufficient for an in vitro reconstituted transcription assay in the presence of 34-kDa subunit; 100 units are sufficient for a protein-protein interaction assay.
EndotoxinNot Tested
Formulation20 mM Tris-Cl (pH 7.9) 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
Concentration200 µg/ml
ReconsitutionAlready in liquid form.
Storage-80 °C
ShippingDry Ice
Background
GenBank Accession #: NM_005513
Amino Acid Sequence:
MADPDVLTEV PAALKRLAKY VIRGFYGIEH ALALDILIRN SCVKEEDMLE LLKFDRKQLR SVLNNLKGDK FIKCRMRVET AADGKTTRHN YYFINYRTLV NVVKYKLDHM RRRIETDERD STNRASFKCP VCSSTFTDLE ANQLFDPMTG TFRCTFCHTE VEEDESAMPK KDARTLLARF NEQIEPIYAL LRETEDVNLA YEILEPEPTE IPALKQSKDH AATTAGAASL AGGHHREAWA TKGPSYEDLY TQNVVINMDD QEDLHRASLE GKSAKERPIW LRESTVQGAY GSEDMKEGGI DMDAFQEREE GHAGPDDNEE VMRALLIHEK KTSSAMAGSV GAAAPVTAAN GSDSESETSE SDDDSPPRPA AVAVHKREED EEEDDEFEEV ADDPIVMVAG RPFSYSEVSQ RPELVAQMTP EEKEAYIAMG QRMFEDLFE
Protein Image