GAL4-VP16 (S. cerevisiae GAL4 [1-147] fused with HSV2 VP16 [411-490]

GAL4-VP16 (S. cerevisiae GAL4 [1-147] fused with HSV2 VP16 [411-490]

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OP10191 GAL4-VP16 (S. cerevisiae GAL4 [1-147] fused with HSV2 VP16 [411-490] 10µg $271 Immediate Add To Shopping Cart
OP10192 GAL4-VP16 (S. cerevisiae GAL4 [1-147] fused with HSV2 VP16 [411-490] 25µg $541 Immediate Add To Shopping Cart
Other VersionsNone
Datasheet
DescriptionThe GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5'-CGGN5(T/A)N5CCG-3' . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription . Herpes virus VP16 activates expression of immediate early genes in virally-infected cells . As most other eukaryotic transcriptional activator proteins, VP16 has a modular domain structure: it’s N-terminus is involved in DNA-protein interactions, while it’s C-terminal 79 amino acids have proven to be an especially potent transactivation domain . When fused to the DNA-binding domain of the yeast GAL4, this VP16 fragment functions as an activator of transcription in yeast, mammalian cells and in vitro transcription assays . VP16 has been shown to bind to TBP , TFIIB , and replication factor A .
Gene ID855828/1487335
Gene NameGAL4 /HS2VP16A
AliasNA
Host SpeciesSaccharomyces cerevisiae /Human herpesvirus 2
Production HostE.coli
TagsHis Tag
Purity> 95% as determined by SDS-PAGE
Bio Activity1 unit equals 1 nanogram of purified protein. 20 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
EndotoxinNot Tested
Formulation20 mM Tris-Cl (pH 7.9) 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
Concentration500 µg/ml
ReconsitutionAlready in liquid form.
Storage-80 °C
ShippingDry Ice
Background
GenBank Accession #: NM_001184062/M60050
Amino Acid Sequence:
MHHHHHHHGG MKLLSSIEQA CDICRLKKLK CSKEKPKCAK CLKNNWECRY SPKTKRSPLT RAHLTEVESR LERLEQLFLL IFPREDLDMI LKMDSLQDIK ALLTGLFVQD NVNKDAVTDR LASVETDMPL TLRQHRISAT SSSEESSNKG QRQLTVSLSA GQRPRRLSTT APITDVSLGD ELRLDGEEVD MTPADALDDF DLEMLGDVES PSPGMTHDPV SYGALDVDDF EFEQMFTDAM GIDDFGG
Protein Image