Human DR1/NC2B Protein, Made in E.coli, with His Tag

Human DR1/NC2B Protein, Made in E.coli, with His Tag

SKU Description Size Price Shipping  
OP10241 Human DR1/NC2B Protein, Made in E.coli, with His Tag 10µg $257 Immediate Add To Shopping Cart
OP10242 Human DR1/NC2B Protein, Made in E.coli, with His Tag 25µg $513 Immediate Add To Shopping Cart
Other VersionsSame Protein with GST Tag
Biotin-labeled and heavy-labeled (C13 and N15) versions of this protein are also available. Please contact us for more information.
Datasheet
DescriptionDr1 is a general negative regulator of class II and class III gene expression . It binds to the basic repeat domain of TBP on promoter DNA and can prevent the RNA polymerase II holoenzyme, or its TFIIB and/or TFIIA subunits, from assembling into an initiation complex . Dr1 is phosphorylated in vivo and this modification affects its interaction with TBP . In addition, Dr1 interacts with the hyperphosphorylated form of Pol II and with the repression domain of the AREB6 repressor . Dr1 forms a heterodimer complex with DRAP1 through its histone fold domain . More recently it was discovered that Dr1-DRAP1 is a bi-functional basal transcription factor that differentially regulates gene transcription through DPE (downstream promoter elements) or TATA box motifs . It can stimulate transcription in vitro from Drosophila promoters containing DPEs, whereas it represses transcription from TATA-containing promoters .
Gene ID1810
Gene NameDR1
AliasNC2, NC2-BETA
Host SpeciesHuman
Production HostE.coli
TagsHis Tag
Purity> 95% as determined by SDS-PAGE
Bio Activity1 unit equals 1 nanogram of purified protein. 20 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
EndotoxinNot Tested
Formulation20 mM Tris-Cl (pH 7.9) 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
Concentration450 µg/ml
ReconsitutionAlready in liquid form.
Storage-80 °C
ShippingDry Ice
Background
GenBank Accession #: NM_001938
Amino Acid Sequence:
MASSSGNDDD LTIPRAAINK MIKETLPNVR VANDARELVV NCCTEFIHLI SSEANEICNK SEKKTISPEH VIQALESLGF GSYISEVKEV LQECKTVALK RRKASSRLEN LGIPEEELLR QQQELFAKAR QQQAELAQQE WLQMQQAAQQ AQLAAASASA SNQAGSSQDE EDDDDI
Protein Image