Human E2F1 Protein, Made in Sf21 Insect Cell, with His Tag

Human E2F1 Protein, Made in Sf21 Insect Cell, with His Tag

SKU Description Size Price Shipping  
OP10311 Human E2F1 Protein, Made in Sf21 Insect Cell, with His Tag 5µg $409 Immediate Add To Shopping Cart
OP10312 Human E2F1 Protein, Made in Sf21 Insect Cell, with His Tag 12.5µg $818 Immediate Add To Shopping Cart
Other VersionsBiotin-labeled and heavy-labeled (C13 and N15) versions of this protein are also available. Please contact us for more information.
Datasheet
DescriptionE2F was originally identified as a transcription factor mediating the transcription of adenovirus E2 gene. It is a heterodimer composed of two structurally related subunits, termed E2F and DP. E2F is encoded by at least five genes, E2F-1 through E2F-5, and DP is encoded by at least two genes, DP-1 and DP-2. All these genes (E2F-1 through E2F-5, DP-1 and DP-2) have been considered as members of the E2F gene family because they have a number of common structural and functional characteristics . Among those members of the E2F family, E2F-1 was the first E2F gene cloned and characterized. The E2F-1 protein has been shown to interact with RB both in vitro and in vivo . Although the E2F proteins can function as transcription factors by themselves, they should be considered as RB-mediators as well because almost every functional response of RB protein(s) requires the presence of E2F protein(s).
Gene ID189971
Gene NameE2F1
AliasE2F-1, RBAP1
Host SpeciesHuman
Production HostSf21 Insect Cell
TagsHis Tag
Purity> 95% as determined by SDS-PAGE
Bio Activity1 unit equals 1 nanogram of purified protein. 1 unit is the amount sufficient for a gel mobility shift assay in a 20µl reaction. 10 units are sufficient for a 25µl reconstituted transcription reaction and 100 units are sufficient for a protein-protein interaction assay detected by an immuno-blot system.
EndotoxinNot Tested
Formulation20 mM Tris-Cl (pH 7.9) 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
Concentration400 µg/ml
ReconsitutionAlready in liquid form.
Storage-80 °C
ShippingDry Ice
Background
GenBank Accession #: NM_005225
Amino Acid Sequence:
MALAGAPAGG PCAPALEALL GAGALRLLDS SQIVIISAAQ DASAPPAPTG PAAPAAGPCD PDLLLFATPQ APRPTPSAPR PALGRPPVKR RLDLETDHQY LAESSGPARG RGRHPGKGVK SPGEKSRYET SLNLTTKRFL ELLSHSADGV VDLNWAAEVL KVQKRRIYDI TNVLEGIQLI AKKSKNHIQW LGSHTTVGVG GRLEGLTQDL RQLQESEQQL DHLMNICTTQ LRLLSEDTDS QRLAYVTCQD LRSIADPAEQ MVMVIKAPPE TQLQAVDSSE NFQISLKSKQ GPIDVFLCPE ETVGGISPGK TPSQEVTSEE ENRATDSATI VSPPPSSPPS SLTTDPSQSL LSLEQEPLLS RMGSLRAPVD EDRLSPLVAA DSLLEHVRED FSGLLPEEFI SLSPPHEALD YHFGLEEGEG IRDLFDCDFG DLTPLDF
Protein Image