Human CTF1 Protein, Made in Sf21 Insect Cell, with His Tag

Human CTF1 Protein, Made in Sf21 Insect Cell, with His Tag

SKU Description Size Price Shipping  
OP10351 Human CTF1 Protein, Made in Sf21 Insect Cell, with His Tag 5µg $409 Immediate Add To Shopping Cart
OP10352 Human CTF1 Protein, Made in Sf21 Insect Cell, with His Tag 12.5µg $818 Immediate Add To Shopping Cart
Other VersionsSame Protein made in E. Coli
Biotin-labeled and heavy-labeled (C13 and N15) versions of this protein are also available. Please contact us for more information.
Datasheet
DescriptionThe CCAAT-box binding protein 1 (CTF1, also called NF-I or NF-1) is a proline-rich transcription activator . It selectively activates transcription of genes that contain GCCAAT consensus sequence in promoter region, including human Ha-Ras, alpha-globin, hsp70 and many other cellular and viral genes (1, 3-5). Cloning and functional characterization of the protein revealed that CTF1 contains an N-terminal DNA binding domain and a C-terminal proline-rich activation domain . CTF1 was shown to interact with the general transcription factor IIB (TFIIB) and facilitate the recruitment of TFIIB to TBP-DNA complexes , with the forkhead thyroid transcription factor TTF2 to regulate hormone–dependent transcription of thyroperoxidase gene , as well as other receptor proteins to synergize hormone receptor-responsive activation.
Gene ID1489
Gene NameCTF-1
AliasCTF/NFI ,
Host SpeciesHuman
Production HostSf21 Insect Cell
TagsHis Tag
Purity> 95% as determined by SDS-PAGE
Bio Activity1 unit equals 1 nanogram purified protein. 30-100 units (ng) are required for a reconstituted transcription assay and 5-20 units (ng) are required for a gel mobility shift assay in a 20µl reaction.
EndotoxinNot Tested
Formulation20 mM Tris-Cl (pH 7.9) 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
Concentration500 µg/ml
ReconsitutionAlready in liquid form.
Storage-80 °C
ShippingDry Ice
Background
GenBank Accession #: X12492
Amino Acid Sequence:
MDEFHPFIEA LLPHVRAFAY TWFNLQARKR KYFKKHEKRM SKDEERAVKD ELLGEKPEVK QKWASRLLAK LRKDIRPECR EDFVLSITGK KAPGCVLSNP DQKGKMRRID CLRQADKVWR LDLVMVILFK GIPLESTDGE RLVKAAQCGH PVLCVQPHHI GVAVKELDLY LAYFVRERDA EQSGSPRTGM GSDQEDSKPI TLDTTDFQES FVTSGVFSVT ELIQVSRTPV VTGTGPNFSL GELQGHLAYD LNPASTGLRR TLPSTSSSGS KRHKSGSMEE DVDTSPGGDY YTSPSSPTSS SRNWTEDMEG GISSPVKKTE MDKSPFNSPS PQDSPRLSSF TQHHRPVIAV HSGIARSPHP SSALHFPTTS ILPQTASTYF PHTAIRYPPH LNPQDPLKDL VSLACDPASQ QPGPLNGSGQ LKMPSHCLSA QMLAPPPPGL PRLALPPATK PATTSEGGAT SPTSPSYSPP DTSPANRSFV GLGPRDPAGI YQAQSWYLG
Protein Image