Bovine HMGB1 Protein, Purified from Calf Thymus

Bovine HMGB1 Protein, Purified from Calf Thymus

SKU Description Size Price Shipping  
OP10581 Bovine HMGB1 Protein, Purified from Calf Thymus 10µg $341 Immediate Add To Shopping Cart
OP10582 Bovine HMGB1 Protein, Purified from Calf Thymus 25µg $680 Immediate Add To Shopping Cart
Other VersionsHMGB1 produced by HEK293, with C-His tag
HMGB1 produced by E.Coli, with C-His tag
HMGB1 purified from Calf Thymus
Biotin-labeled and heavy-labeled (C13 and N15) versions of this protein are also available. Please contact us for more information.
Datasheet
DescriptionHigh mobility group 1 (HMG1) is a 26 kDa highly conserved non-sequence-specific DNA-binding nuclear protein . Mammalian HMG1 has two homologous DNA-binding domains HMG boxes, A and B (each of 80–90 amino-acid residues), linked by a short basic region to an acidic C-terminal domain containing 30 consecutive Asp and Glu residues . HMG1 has been implicated in a number of fundamental biological processes including transcription, replication and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compaction or unwinding, or by directly contacting with distinct cellular proteins . HMG-1 can act as a repressor, by interacting with TBP to block pre-initiation complex formation or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences . Most recently, it was discovered that HMG-1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release .
Gene ID282691
Gene NameHMGB1
AliasHMG1
Host SpeciesBos taurus
Production HostCalf Thymus
TagsNo Tag
Purity> 95% as determined by SDS-PAGE
Bio Activity1 unit equals 1 nanogram of purified protein. 10-100 units are required for a gel mobility shift assay in a 20µl reaction to super-shift TBP-DNA complex, 50-500 units are required for a reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
EndotoxinNot Tested
Formulation20 mM Tris-Cl (pH 7.9) 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
Concentration900 µg/ml
ReconsitutionAlready in liquid form.
Storage-80 °C
ShippingDry Ice
Background
GenBank Accession #: X12796
Amino Acid Sequence:
MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEEEEE DDDDE
Protein Image