Human NCOA1/SRC1 Protein (Receptor Interaction Domain [AA627-786]), Made in E.coli, with Flag Tag (TEV cleavable)

Human NCOA1/SRC1 Protein (Receptor Interaction Domain [AA627-786]), Made in E.coli, with Flag Tag (TEV cleavable)

SKU Description Size Price Shipping  
OP10951 Human NCOA1/SRC1 Protein (Receptor Interaction Domain [AA627-786]), Made in E.coli, with Flag Tag (TEV cleavable) 10µg $244 Immediate Add To Shopping Cart
OP10952 Human NCOA1/SRC1 Protein (Receptor Interaction Domain [AA627-786]), Made in E.coli, with Flag Tag (TEV cleavable) 25µg $488 Immediate Add To Shopping Cart
Other VersionsNCOA1 with No Tag
NCOA1 with No Tag, Biotinylated
NCOA1 with His Tag
NCOA1 with His Tag, Biotinylated
NCOA1 with His Tag (TEV Cleavable)
NCOA1 with FLAG Tag (TEV cleavable)
NCOA1 with GST Tag
Datasheet
DescriptionSteroid receptor coactivator 1 (SRC1) is a transcriptional coactivator that mediates the activating functions of many of the nuclear hormone receptors. It is also known as NCoA1 and is a member of the SRC/p160 coactivator family . SRC1 has been shown to be over expressed in some cancers . SRC1 is a 160 kDa protein that contains several LXXLL motifs, which are involved in nuclear receptor interaction . The region 627-786 contains 3 LXXLL motifs that are involved in interaction with nuclear hormone receptors and have been previously used in assays detecting ligand-dependent receptor-cofactor interactions . This protein contains the Tobacco Etch virus (TEV) protease site and can be cleaved using acTEV to yield untagged SRC1 RID. This protein will be useful for assays where a native untagged protein is desired.
Gene ID8648
Gene NameNCOA1
AliasRIP160, SRC1
Host SpeciesHuman
Production HostE.coli
TagsFlag Tag
Purity> 95% as determined by SDS-PAGE
Bio Activity1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay.
EndotoxinNot Tested
Formulation20 mM Tris-Cl (pH 7.9) 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
Concentration1000 µg/ml
ReconsitutionAlready in liquid form.
Storage-80 °C
ShippingDry Ice
Background
GenBank Accession #: NM_003743
Amino Acid Sequence:
SQTSHKLVQL LTTTAEQQLR HADIDTSCKD VLSCTGTSNS ASANSSGGSC PSSHSSLTER HKILHRLLQE GSPSDITTLS VEPDKKDSAS TSVSVTGQVQ GNSSIKLELD ASKKKESKDH QLLRYLLDKD EKDLRSTPNL SLDDVKVKVE KKEQMDPCNT
Protein Image