Human SRSF1/ASF/SF2/SFRS1 Protein, Made in E.coli, with His Tag

Human SRSF1/ASF/SF2/SFRS1 Protein, Made in E.coli, with His Tag

SKU Description Size Price Shipping  
OP30011 Human SRSF1/ASF/SF2/SFRS1 Protein, Made in E.coli, with His Tag 10µg $284 Immediate Add To Shopping Cart
OP30012 Human SRSF1/ASF/SF2/SFRS1 Protein, Made in E.coli, with His Tag 25µg $566 Immediate Add To Shopping Cart
Other VersionsSRSF1 produced from E.Coli
SRSF1 produced from Sf21
Datasheet
DescriptionASF or called SF2, a member of the SR protein family, is an essential pre-mRNA splicing factor required for both single and alternative splicing . Phosphorylation on serine residues located within the SR domain directly regulates ASF/SF2 activity and compartmentalization of other SR splicing factors . In addition to interacting with RNA and other splicing factors, such as U1-70K, U2AF and other SR proteins, ASF/SF2 also directly or indirectly interacts with HIV regulatory protein Rev, the C-terminal domain (CTD) of the largest subunit of RNA polymerase II, and numerous transcription factors, thereby suggesting a potential role of ASF/SF2 in coordinating of transcription and pre-mRNA splicing .
Gene ID6426
Gene NameSRSF1
AliasASF, SF2
Host SpeciesHuman
Production HostE.coli
TagsHis Tag
Purity> 95% as determined by SDS-PAGE
Bio Activity1 unit equals 1 nanogram of purified protein. 1-5 units are sufficient for a gel mobility shift assay in a 20 µl reaction, 100 units are sufficient for a protein-protein interaction assay and 100-500 units are required for a splicing assay in a 20 µl reaction.
EndotoxinNot Tested
Formulation20 mM Tris-Cl (pH 7.9) 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
Concentration650 µg/ml
ReconsitutionAlready in liquid form.
Storage-80 °C
ShippingDry Ice
Background
GenBank Accession #: NM_006924
Amino Acid Sequence:
SGGGVIRGPA GNNDCRIYVG NLPPDIRTKD IEDVFYKYGA IRDIDLKNRR GGPPFAFVEF EDPRDAEDAV YGRDGYDYDG YRLRVEFPRS GRGTGRGGGG GGGGGAPRGR YGPPSRRSEN RVVVSGLPPS GSWQDLKDHM REAGDVCYAD VYRDGTGVVE FVRKEDMTYA VRKLDNTKFR SHEGETAYIR VKVDGPRSPS YGRSRSRSRS RSRSRSRSNS RSRSYSPRRS RGSPRYSPRH SRSRSRT
Protein Image