Thermostable FGF2 Protein Expressed from HEK293 Stable Cell Line

Thermostable FGF2 Protein Expressed from HEK293 Stable Cell Line

SKU Description Size Price Shipping  
SDP1025 Thermostable FGF2 Protein Expressed from HEK293 Stable Cell Line 10 μg $129 2 Days Add To Shopping Cart
SDP1026 Thermostable FGF2 Protein Expressed from HEK293 Stable Cell Line 50 μg $582 2 Days Add To Shopping Cart
SDP1027 Thermostable FGF2 Protein Expressed from HEK293 Stable Cell Line 1 mg $3232 2 Days Add To Shopping Cart
Other VersionsHuman FGF2 (Pro143-Ser288), Tag-free, expressed from E.coli
Rat FGF2 / FGFb, Tag-free, expressed from E.coli
Authentic Human FGF2/FGF-2/bFGF/FGF Basic Expressed from HEK293 Stable Cell Line
Human FGF2 (Gly132-Ser288), Tag-free, expressed from E.coli
Human FGF2 (Met134-Ser288), Tag-free, expressed from E.coli
Mouse FGF2, Tag-free, expressed from E.coli
Biotin-labeled and heavy-labeled (C13 and N15) versions of this protein are also available. Please contact us for more information.
Datasheet
DescriptionThis protein is one of our premium HumanKine Cytokines and Growth Factors expressed in human 293 cells. FGF basic is a heparin-binding growth factor and critical for human embryonic stem cells to remain in an undifferentiated state during cell culture. This thermostable version of FGF Basic is specially engineered to enhance its thermostability without affecting its biological functions. FGFbasic-TS consists of 154 amino acids. It is a 17 kDa non-glycosylated monomer. Please Click here for more information regarding this protein.
Gene ID2247
Gene NameFGF2
AliasBFGF, FGFB, FGF-2, HBGF-2, fibroblast growth factor 2 (basic)
Host SpeciesHuman
Production HostHEK293 stable cell line
TagsNative protein, contains no tags
Purity> 95%, determined by SDS-PAGE
Bio Activity0.007 ng/ml ED50. The specific activity was determined by the dose-dependent stimulation of the proliferation of the 3T3 cell line.
EndotoxinThe protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
FormulationThe protein was lyophilized after filtrated through a 0.22 um filter. Before lyophilization, the protein was in PBS buffer.
ConcentrationLyophilized Powder.
ReconsitutionReconstitute lyophilized powder with PBS to no less than 100 ug/ml, which can be further diluted in other desired aqueous solutions. It is recommended that 0.1% rHSA (SDP1139) as a carrier protein if concentration is less than 100 ug/ml.
StorageLyophilized form is stable at room temperature for 3 weeks. Keep desiccated at below -18C for long term storage. After reconstituted, store at 4C for 2-7 days or at below -18C for up to 3 months.
ShippingShip at ambient conditions.
Background
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq]
Protein Image
Activity assay plot (left) and SDS-PAGE analysis (right).