Recombinant rabbit anti-human SIRPA monoclonal antibody, clone DM8

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PRODUCT

  • SKU

    DMR1349-100ug

  • Size

    100 ug

  • Price

    $669

1
  • SKU

    DMR1349-500ug

  • Size

    500 ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target SIRPA
alias BIT, CD172a, MFR, MYD-1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2
gene id Uniprot ID: P78324
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM8
iso type Rabbit IgG
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA, Flow Cytometry, IF, IP
dilution Flow Cyt 1:100; IP 1:30
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

THP-1 cell line were surface stained with Rabbit anti-SIRPα monoclonal antibody 1ug/ml (clone: DM8) and Rabbit IgG isotype control antibody followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

A) Flow cytometry data of serially titrated Rabbit anti-SIRPα monoclonal antibody (clone: DM8). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. B) Competition assay demonstrating DM8 blockade of CD47 binding to THP-1 cells. IC50=30.88ng/ml.


PRODUCT

  • SKU

    DMR1349-100ug

  • Size

    100 ug

  • Price

    $669

1
  • SKU

    DMR1349-500ug

  • Size

    500 ug

  • Price

    $2,010

1