Recombinant rabbit anti-human TPBG/5T4 monoclonal antibody, clone DM139

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PRODUCT

  • SKU

    DMR1416-100ug

  • Size

    100 ug

  • Price

    $669

1
  • SKU

    DMR1416-500ug

  • Size

    500 ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target TPBG
alias 5T4
gene id Uniprot ID: Q13641
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number DM139
iso type Rabbit IgG
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications ELISA, Flow Cytometry
dilution ELISA 1:5000-10000; Flow Cyt 1:100
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 1 ug/ml (100 ul/well) Human 5T4 protein, His tagged protein binds to Rabbit anti-5T4 monoclonal antibody (clone: DM139) in a linear range of 0.1-16 ng/ml.

5T4 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis of anti-5T4 (DM139) on Expi293 cells transfected with human 5T4 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram),and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram)


PRODUCT

  • SKU

    DMR1416-100ug

  • Size

    100 ug

  • Price

    $669

1
  • SKU

    DMR1416-500ug

  • Size

    500 ug

  • Price

    $2,010

1