Human CCR4 Full Length Transmembrane Protein (MNP, Membrane Nanoparticle)

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PRODUCT

  • SKU

    DMF1013-50ug

  • Size

    50 ug

  • Price

    $1,800

1
  • SKU

    DMF1013-100ug

  • Size

    100 ug

  • Price

    $2,400

1
Cell membrane coated nanoparticles (MNPs) harboring target full-length multi-transmembrane protein in its native form were produced by overexpression of the protein in HEK293 cells followed by membrane extraction and extrusion. The target transmembrane protein maintains its natural conformation embedded in natural cell membrane coated on nanoparticles.
This specific membrane protein, the human full length CCR4 protein (Uniprot ID: P51679), has a MW of 41.4 kDa.
gene name CCR4
alias CC-CKR-4, CD194, ChemR13, CKR4, CMKBR4
gene id Uniprot ID: P51679
host species Human
production host HEK293
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose was added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

Elisa plates were pre-coated with 0.5ug/well purified human CCR4 full length membrane nanoparticles. Serial diluted anti-CCR4 monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. Calculated from the data, EC50 for anti-CCR4 monoclonal antibody binding with CCR4 full length membrane nanoparticles is 308.3ng/ml.

FACS analysis of CCR4 MNPs A. Negative Control 1: CCR4 full length membrane nanoparticles samples were stained only with Goat anti-human lgG 488 secondary antibody. B. Negative Control 2: Control membrane nanoparticles samples were stained with anti-CCR4 antibody at 2ug/mL, followed by Goat anti-human IgG 488 secondary antibody. C. Negative Control 3: CCR4 full length membrane nanoparticles samples were stained with anti-CCR8 antibody (an irrelevant antibody) at 2ug/mL, followed by Goat anti-human IgG 488 secondary antibody. D. CCR4 full length membrane nanoparticles samples were stained with anti-CCR4 antibody at 2ug/mL, followed by Goat anti-human IgG 488 secondary antibody.


PRODUCT

  • SKU

    DMF1013-50ug

  • Size

    50 ug

  • Price

    $1,800

1
  • SKU

    DMF1013-100ug

  • Size

    100 ug

  • Price

    $2,400

1