Human CD63 Full Length Transmembrane Protein (MNP, Membrane Nanoparticle)

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PRODUCT

  • SKU

    DMF1017-50ug

  • Size

    50 ug

  • Price

    $1,800

1
  • SKU

    DMF1017-100ug

  • Size

    100 ug

  • Price

    $2,400

1
Cell membrane coated nanoparticles (MNPs) harboring target full-length multi-transmembrane protein in its native form were produced by overexpression of the protein in HEK293 cells followed by membrane extraction and extrusion. The target transmembrane protein maintains its natural conformation embedded in natural cell membrane coated on nanoparticles.
This specific membrane protein, the human full length CD63 protein (Uniprot ID: P08962), has a MW of 25.6 kDa.
gene name CD63
alias LAMP-3, ME491, MLA1, OMA81H, TSPAN30
gene id Uniprot ID: P08962
host species Human
production host HEK293
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose was added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

Elisa plates were pre-coated with 0.5ug/well purified human CD63 full length membrane nanoparticles. Serial diluted anti-CD63 monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. Calculated from the data, EC50 for anti-CD63 monoclonal antibody binding with CD63 full length membrane nanoparticles is 61.65ng/ml.

FACS analysis of CD63 MNPs A. Negative Control 1: CD63 full length membrane nanoparticles samples were stained only with Goat anti-human lgG 488 secondary antibody. B. Negative Control 2: Control membrane nanoparticles samples were stained with anti-CD63 antibody at 2ug/mL, followed by Goat anti-human IgG 488 secondary antibody. C. Negative Control 3: CD63 full length membrane nanoparticles samples were stained with anti-GPRC5D antibody (an irrelevant antibody) at 2ug/mL, followed by Goat anti-human IgG 488 secondary antibody. D. CD63 full length membrane nanoparticles samples were stained with anti-CD63 antibody at 2ug/mL, followed by Goat anti-human IgG 488 secondary antibody.


PRODUCT

  • SKU

    DMF1017-50ug

  • Size

    50 ug

  • Price

    $1,800

1
  • SKU

    DMF1017-100ug

  • Size

    100 ug

  • Price

    $2,400

1