Human CALCRL / CGRPR-RAMP1 Full Length Transmembrane Protein (MNP, Membrane Nanoparticle)

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PRODUCT

  • SKU

    DMF1018-50ug

  • Size

    50 ug

  • Price

    $1,800

1
  • SKU

    DMF1018-100ug

  • Size

    100 ug

  • Price

    $2,400

1
Cell membrane coated nanoparticles (MNPs) harboring target full-length multi-transmembrane protein in its native form were produced by overexpression of the protein in HEK293 cells followed by membrane extraction and extrusion. The target transmembrane protein maintains its natural conformation embedded in natural cell membrane coated on nanoparticles.
This specific membrane protein, the human full length CGRPR protein (Uniprot ID: Q16602), has a MW of 53.0 kDa.
gene name CALCRL
alias CGRPR, CRLR, LMPHM8
gene id Uniprot ID: Q16602
host species Human
production host HEK293
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose was added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

Elisa plates were pre-coated with 0.5ug/well purified human CGRPR-RAMP1 full length membrane nanoparticles. Serial diluted anti-CGRPR monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. Calculated from the data, EC50 for anti-CGRPR monoclonal antibody binding with CGRPR-RAMP1 full length membrane nanoparticles is 122.8ng/ml.


PRODUCT

  • SKU

    DMF1018-50ug

  • Size

    50 ug

  • Price

    $1,800

1
  • SKU

    DMF1018-100ug

  • Size

    100 ug

  • Price

    $2,400

1