Home/ Products / Full-length Membrane Proteins
DMF1038-10ug
10 ug
$1,350
DMF1038-50ug
50 ug
$6,600
DMF1038-100ug
100 ug
$12,000
Synthetic nanodiscs were produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs were further purified, offering intact membrane proteins in their natural configuration.
This specific membrane protein, the human full length GPR75 protein (Uniprot ID: O95800), has a MW of 59.4 kDa.
| gene name | GPR75 |
| alias | GPRchr2, WI31133 |
| gene id | Uniprot ID: O95800 |
| host species | Human |
| production host | HEK293 |
| formulation | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose was added as protectants before lyophilization. |
| reconstitution | Reconstitute lyophilized powder with 19.23 uL of water, solubilize with gentle tapping for 5-10 times. |
| shipping | Shipped at ambient conditions. |
| storage | Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months. |
Elisa plates were pre-coated with Flag Tag GPR75-Nanodisc (0.2ug/per well). Serial diluted anti-GPR75 monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. Calculated from the data, EC50 for anti-GPR75 monoclonal antibody binding with GPR75-Nanodisc is 303.6ng/ml.
WB analysis of Human GPR75-Nanodisc with anti-Flag monoclonal antibody at 1/5000 dilution, followed by Goat anti-Rabbit IgG HRP at 1/5000 dilution
DMF1038-10ug
10 ug
$1,350
DMF1038-50ug
50 ug
$6,600
DMF1038-100ug
100 ug
$12,000