Human AGTR1 Full Length Transmembrane Protein (Synthetic Nanodisc) with N-MBP and C-Flag Tag

Home/ Products / Full-length Membrane Proteins

PRODUCT

  • SKU

    DMF1507-10ug

  • Size

    10 ug

  • Price

    $1,350

1
  • SKU

    DMF1507-50ug

  • Size

    50 ug

  • Price

    $6,600

1
  • SKU

    DMF1507-100ug

  • Size

    100 ug

  • Price

    $12,000

1
Synthetic nanodiscs are produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs are further purified, offering intact membrane proteins in their natural configuration. This human full length MBP-AGTR1 protein has a MW of 81.1 kDa.
target AGTR1
gene name AGTR1
alias AG2S, AGTR1B, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, HAT1R
gene id Uniprot ID: P30556
tags N-MBP Tag, C-Flag Tag
conjugate None
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

Elisa plates were pre-coated with N-MBP Tag, C-Flag Tag MBP-AGTR1-Nanodisc (0.2 ug/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with MBP-AGTR1-Nanodisc is 4.309 ng/mL.

Human MBP-AGTR1-Nanodisc with N-MBP Tag and C-Flag Tag on SDS-PAGE


PRODUCT

  • SKU

    DMF1507-10ug

  • Size

    10 ug

  • Price

    $1,350

1
  • SKU

    DMF1507-50ug

  • Size

    50 ug

  • Price

    $6,600

1
  • SKU

    DMF1507-100ug

  • Size

    100 ug

  • Price

    $12,000

1