Human GP119 / GPR119 Full Length Transmembrane Protein (Synthetic Nanodisc) with Flag-Strep Tag

Home/ Products / Full-length Membrane Proteins

PRODUCT

  • SKU

    DMF1744-10ug

  • Size

    10 ug

  • Price

    $1,215

1
  • SKU

    DMF1744-50ug

  • Size

    50 ug

  • Price

    $5,940

1
  • SKU

    DMF1744-100ug

  • Size

    100 ug

  • Price

    $10,800

1
Synthetic nanodiscs are produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs are further purified, offering intact membrane proteins in their natural configuration. This human full length GP119-Strep protein has a MW of 36.9 kDa.
target GP119
gene name GPR119
alias GPCR2
gene id Uniprot ID: Q8TDV5
tags C-Flag&Strep Tag
conjugate None
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

Elisa plates were pre-coated with C-Flag&Strep Tag GPR119-Strep-Nanodisc (0.2 ug/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GPR119-Strep-nanodisc is 1.841 ng/mL.

Human GPR119-Strep-Nanodisc (Flag-Strep Tag) on SDS-PAGE


PRODUCT

  • SKU

    DMF1744-10ug

  • Size

    10 ug

  • Price

    $1,215

1
  • SKU

    DMF1744-50ug

  • Size

    50 ug

  • Price

    $5,940

1
  • SKU

    DMF1744-100ug

  • Size

    100 ug

  • Price

    $10,800

1