Human GPER1 Full Length Transmembrane Protein (Synthetic Nanodisc) with Flag-Strep Tag

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PRODUCT

  • SKU

    DMF1773-10ug

  • Size

    10 ug

  • Price

    $1,215

1
  • SKU

    DMF1773-50ug

  • Size

    50 ug

  • Price

    $5,940

1
  • SKU

    DMF1773-100ug

  • Size

    100 ug

  • Price

    $10,800

1
Synthetic nanodiscs are produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs are further purified, offering intact membrane proteins in their natural configuration. This human full length GPER1-Strep protein has a MW of 42.2 kDa.
target GPER1
gene name GPER1
alias CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPER, GPR30, LERGU, LERGU2, LyGPR, mER
gene id Uniprot ID: Q99527
tags C-Flag&Strep Tag
conjugate None
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

Elisa plates were pre-coated with C-Flag&Strep Tag GPER1-Strep-Nanodisc (0.2 ug/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GPER1-Strep-nanodisc is 5.129 ng/mL.

Human GPER1-Strep-Nanodisc (Flag-Strep Tag) on SDS-PAGE

PRODUCT

  • SKU

    DMF1773-10ug

  • Size

    10 ug

  • Price

    $1,215

1
  • SKU

    DMF1773-50ug

  • Size

    50 ug

  • Price

    $5,940

1
  • SKU

    DMF1773-100ug

  • Size

    100 ug

  • Price

    $10,800

1

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