Human ACKR3 / CXCR7 Full Length Transmembrane Protein (Synthetic Nanodisc) with C-Flag and Avi Tag, Biotinylated

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PRODUCT

  • SKU

    DMF2237-10ug

  • Size

    10ug

  • Price

    $1,485

1
  • SKU

    DMF2237-50ug

  • Size

    50ug

  • Price

    $7,260

1
  • SKU

    DMF2237-100ug

  • Size

    100ug

  • Price

    $13,200

1
Synthetic nanodiscs are produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs are further purified, offering intact membrane proteins in their natural configuration. This human full length CXCR7 Protein has a MW of 44.4 kDa.
target CXCR7
gene name ACKR3
alias RDC1; ACKR3; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159
gene id Uniprot ID: P25106
tags C-Flag and Avi Tag
conjugate Biotinylated
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

ELISA plate pre-coated by 2 ug/mL (100 uL/well) Anti-Flag Rabbit mAb can bind Biotinylated Human CXCR7 full length protein-synthetic nanodisc in a linear range of 16?400 ng/ml.HRP Conjugated Streptavidin was used as detection antibody.

ELISA plate pre-coated by 2 ug/mL (100 uL/well) Anti-CXCR7 antibody can bind Biotinylated Human CXCR7 full length protein-synthetic nanodisc in a linear range of 16?400 ng/ml.HRP Conjugated Streptavidin was used as detection antibody.

ELISA plate pre-coated by 2 ug/mL (100 uL/well) Biotinylated Human CXCR7 full length protein-synthetic nanodisc can bind Anti-Flag Rabbit mAb in a linear range of 0.64-16 ng/mL.

ELISA plate pre-coated by 1 ug/mL (100 uL/well) Streptavidin can bind Biotinylated Human CXCR7 full length protein-synthetic nanodisc in a linear range of 16-400 ng/ml.Anti-Flag Rabbit antibody was used as detection antibody.

Biotinylated Human CXCR7-Nanodisc (Flag Tag) on SDS-PAGE

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PRODUCT

  • SKU

    DMF2237-10ug

  • Size

    10ug

  • Price

    $1,485

1
  • SKU

    DMF2237-50ug

  • Size

    50ug

  • Price

    $7,260

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  • SKU

    DMF2237-100ug

  • Size

    100ug

  • Price

    $13,200

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