Human GPR21 Full Length Transmembrane Protein (Synthetic Nanodisc) with C-Flag Tag

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PRODUCT

  • SKU

    DMF2240-10ug

  • Size

    10ug

  • Price

    $1,350

1
  • SKU

    DMF2240-50ug

  • Size

    50ug

  • Price

    $6,600

1
  • SKU

    DMF2240-100ug

  • Size

    100ug

  • Price

    $12,000

1
Synthetic nanodiscs are produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs are further purified, offering intact membrane proteins in their natural configuration. This human full length GPR21 protein has a MW of 39.5 kDa
target GPR21
gene name GPR21
alias N/A
gene id Uniprot ID: Q99679
tags C-Flag Tag
conjugate None
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

Elisa plates were pre-coated with Flag Tag GPR21-Nanodisc (0.2 ug/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GPR21-Nanodisc is 2.219 ng/mL.

Human GPR21-Nanodisc (Flag Tag) on SDS-PAGE


PRODUCT

  • SKU

    DMF2240-10ug

  • Size

    10ug

  • Price

    $1,350

1
  • SKU

    DMF2240-50ug

  • Size

    50ug

  • Price

    $6,600

1
  • SKU

    DMF2240-100ug

  • Size

    100ug

  • Price

    $12,000

1