Home/ Products / Full-length Membrane Proteins
DMF2244-10ug
10ug
$1,485
DMF2244-50ug
50ug
$7,260
DMF2244-100ug
100ug
$13,200
Synthetic nanodiscs are produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs are further purified, offering intact membrane proteins in their natural configuration. This human full length CD19 Protein has a MW of 65.9 kDa.
| target | CD19 |
| gene name | CD19 |
| alias | B4; CVID3 |
| gene id | Uniprot ID: P15391 |
| tags | C-Flag and Avi Tag |
| conjugate | Biotinylated |
| host species | Human |
| production host | HEK293 |
| formulation | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. |
| reconstitution | Reconstitute lyophilized powder with 20 uL of water to a concentration of 500 ug/ml, solubilize with occasional gentle mixing. |
| shipping | Shipped at ambient conditions. |
| storage | Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months. |
ELISA plate pre-coated by 2 ug/mL (100 uL/well) Biotinylated Human CD19 full length protein-synthetic nanodisc can bind Anti-Flag Rabbit mAb in a linear range of 0.13-16 ng/mL.
ELISA plate pre-coated by 1 ug/mL (100 uL/well) Streptavidin can bind Biotinylated Human CD19 full length protein-synthetic nanodisc in a linear range of 80-2000 ng/ml.Anti-Flag Rabbit antibody was used as detection antibody.
ELISA plate pre-coated by 2 ug/mL (100 uL/well) Anti-flag Rabbit mAb can bind Biotinylated Human CD19 full length protein-synthetic nanodisc in a linear range of 80-2000 ng/ml.HRP Conjugated Streptavidin was used as detection antibody.
Biotinylated Human CD19-Nanodisc (Flag Tag) on SDS-PAGE
DMF2244-10ug
10ug
$1,485
DMF2244-50ug
50ug
$7,260
DMF2244-100ug
100ug
$13,200