Synthetic nanodisc negative control

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PRODUCT

  • SKU

    DMF2254-10ug

  • Size

    10ug

  • Price

    $945

1
  • SKU

    DMF2254-50ug

  • Size

    50ug

  • Price

    $4,620

1
  • SKU

    DMF2254-100ug

  • Size

    100ug

  • Price

    $8,400

1

The protein in the nanodisc is a three-part fusion protein. The N-terminal extracellular region is a Flag tag. The C-terminal intracellular region is an EGFP-Strep tag. The transmembrane region of the protein is derived from the transmembrane region of the platelet-derived growth factor receptor beta (PDGFRB). This synthetic nanodisc negative control can be used as a control for membrane protein nanodiscs in various applications.

tags N-Flag&C-Strep Tag
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with 25 ul of water to a concentration of 400 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

PRODUCT

  • SKU

    DMF2254-10ug

  • Size

    10ug

  • Price

    $945

1
  • SKU

    DMF2254-50ug

  • Size

    50ug

  • Price

    $4,620

1
  • SKU

    DMF2254-100ug

  • Size

    100ug

  • Price

    $8,400

1