Home/ Products / Full-length Membrane Proteins
DMF2255-10ug
10ug
$1,485
DMF2255-50ug
50ug
$7,260
DMF2255-100ug
100ug
$13,200
Synthetic nanodiscs are produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs are further purified, offering intact membrane proteins in their natural configuration. This human full length TLR4 protein has a MW of 95.7 kDa.
| target | TLR4 |
| gene name | TLR4 |
| alias | ARMD10, CD284, TLR-4 |
| gene id | Uniprot ID: O00206 |
| tags | C-Flag-Avi Tag |
| conjugate | Biotinylated |
| host species | Human |
| production host | HEK293 |
| formulation | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. |
| reconstitution | Reconstitute lyophilized powder with 30 ul of water to a concentration 333 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments. |
| shipping | Shipped at ambient conditions. |
| storage | Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months. |
SDS-PAGE analysis of biotinylated human TLR4 nanodisc.
Elisa plates were pre-coated with 0.2 ug per wellof Streptavidin then added with this biotinylated human TLR4-Nanodisc (0.2 ug per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with Biotinylated TLR4-Nanodisc is 312.5 ng/ml.
DMF2255-10ug
10ug
$1,485
DMF2255-50ug
50ug
$7,260
DMF2255-100ug
100ug
$13,200