Home/ Products / Full-length Membrane Proteins
DMF2260-10ug
10ug
$1,650
DMF2260-50ug
50ug
$7,986
DMF2260-100ug
100ug
$14,520
| target | LGR5 |
| gene name | LGR5 |
| alias | FEX, GPR49, GPR67, GRP49, HG38 |
| gene id | Uniprot ID: O75473 |
| tags | C-Flag and Avi Tag |
| conjugate | Biotinylated |
| host species | Human |
| production host | HEK293 |
| formulation | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. |
| reconstitution | Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments. |
| shipping | Shipped at ambient conditions. |
| storage | Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months. |
SDS-PAGE anaylsysis of biotinylated LGR5 PeptiNanodisc
Biotinylated LGR5-PeptiNanodisc were added into ELISA wells (0.2 ug/per well) pre-coated with streptavidin (0.2 ug/per well) plate. Serial diluted anti-LGR5 monoclonal antibody and secondary antibody solutions were added, washed, and incubated sequencially before Elisa reading. From above data, the EC50 for anti-LGR5 monoclonal antibody binding with Biotinylated LGR5-PeptiNanodisc is 7.7 ng/ml.
DMF2260-10ug
10ug
$1,650
DMF2260-50ug
50ug
$7,986
DMF2260-100ug
100ug
$14,520