Human CACNG1 Full Length Transmembrane Protein (Synthetic Nanodisc) with C-Flag and Avi Tag, Biotinylated

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PRODUCT

  • SKU

    DMF2261-10ug

  • Size

    10ug

  • Price

    $1,550

1
  • SKU

    DMF2261-50ug

  • Size

    50ug

  • Price

    $7,260

1
  • SKU

    DMF2261-100ug

  • Size

    100ug

  • Price

    $13,200

1
Synthetic nanodiscs are produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs are further purified, offering intact membrane proteins in their natural configuration. This human full length CACNG1 protein has a MW of 25.0 kDa.
target CACNG1
gene name CACNG1
alias CACNLG
gene id Uniprot ID: Q06432
tags C-Flag and Avi Tag
conjugate Biotinylated
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE anaylsysis of CACNG1 full length protein-synthetic nanodisc

Biotinylated CACNG1 nanodisc were added into ELISA wells (0.1 ug/per well) pre-coated with streptavidin (0.1 ug/per well) plate. Serial diluted anti-Flag monoclonal antibody and secondary antibody solutions were added, washed, and incubated sequencially before Elisa reading. From above data, the anti-Flag monoclonal antibody showed linear binding to Flag-tagged biotinylated CACNG1 nanodisc in the range of 1.6 - 8 ng/ml.


PRODUCT

  • SKU

    DMF2261-10ug

  • Size

    10ug

  • Price

    $1,550

1
  • SKU

    DMF2261-50ug

  • Size

    50ug

  • Price

    $7,260

1
  • SKU

    DMF2261-100ug

  • Size

    100ug

  • Price

    $13,200

1