Human SLC2A1 Full Length Transmembrane Protein (Synthetic Nanodisc) with C-Flag and Strep Tag

Home/ Products / Full-length Membrane Proteins

PRODUCT

  • SKU

    DMF2986-10ug

  • Size

    10ug

  • Price

    $1,215

1
  • SKU

    DMF2986-50ug

  • Size

    50ug

  • Price

    $5,940

1
  • SKU

    DMF2986-100ug

  • Size

    100ug

  • Price

    $10,800

1
Synthetic nanodiscs are produced by solubilizing HEK293 cell membrane fractions overexpessing target full-length multi-transmembrane protein with synthetic polymers such as styrene-maleic acid co-polymers (SMAs), forming discoidal nanostructures with target protein intact in lipid bilayer. The nanodiscs are further purified, offering intact membrane proteins in their natural configuration. This human full length SLC2A1-Strep protein has a MW of 54.1 kDa.
target SLC2A1
gene name SLC2A1
alias CSE, DYT9, DYT17, DYT18, EIG12, GLUT, GLUT-1, GLUT1, GLUT1DS, HTLVR, PED, SDCHCN
gene id Uniprot ID: P11166
tags C-Flag and Strep Tag
conjugate None
host species Human
production host HEK293
formulation Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize with occasional gentle mixing. Do not use solvents with pH lower than 6.5 in subsequent experiments.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE anaylsysis of SLC2A1-Strep synthetic nanodisc

Elisa plates were pre-coated with SLC2A1-Strep-Nanodisc (0.2 ug/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with SLC2A1-Strep-nanodisc is 1.744 ng/ml.

PRODUCT

  • SKU

    DMF2986-10ug

  • Size

    10ug

  • Price

    $1,215

1
  • SKU

    DMF2986-50ug

  • Size

    50ug

  • Price

    $5,940

1
  • SKU

    DMF2986-100ug

  • Size

    100ug

  • Price

    $10,800

1

CONTACT US TO ORDER