Human CD27 (Ala20-Arg191) protein, with C-hFc Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP1245-50ug

  • Size

    50 ug

  • Price

    $648

1
  • SKU

    DMP1245-100ug

  • Size

    100 ug

  • Price

    $971

1
This recombinant protein contains Ala20-Arg191 of Uniprot ID P26842. It has a C-hFc Tag, with a calculated molecular weight of 45.3 kDa. In SDS-PAGE, the protein migrates at 55-70 kDa due to glycosylation.
gene name CD27
gene id Uniprot ID: P26842
alias CD27, TNFRSF7, S152, T14, Tp55
host species Human
production host HEK293
tags C-hFc Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

ELISA plate coated with 2 ug/ml (100 ul/well) Human CD27, hFc tagged protein binds to anti-CD27 neutralizing antibody in a linear range of 3.2-80.0 ng/ml.

ELISA plate coated with 2 ug/ml (100 ul/well) Human CD27, hFc tagged protein binds to Human CD70,mFc-His tagged protein in a linear range of 3.2-400 ng/ml.

Flow cytometry analysis of 15 ug/mL Human CD27 Protein, hFc tag on Expi293 cells transfected with human CD70 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Human CD27 Protein, hFc Tag, SDS-PAGE analysis under reducing conditions.


PRODUCT

  • SKU

    DMP1245-50ug

  • Size

    50 ug

  • Price

    $648

1
  • SKU

    DMP1245-100ug

  • Size

    100 ug

  • Price

    $971

1