Human TNFSF13B / BAFF (Ala134-Leu285) protein, with N-hFc Tag, expressed from HEK293

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PRODUCT

  • SKU

    DMP2206-50ug

  • Size

    50ug

  • Price

    $648

1
  • SKU

    DMP2206-100ug

  • Size

    100ug

  • Price

    $971

1
This recombinant protein contains Ala134-Leu285 of Uniprot ID Q9Y275. It has a N-hFc Tag, with a calculated molecular weight of 43.2 kDa. In SDS-PAGE, the protein migrates at 50-55 kDa due to glycosylation.
gene name TNFSF13B
gene id Uniprot ID: Q9Y275
alias TNFSF13B, BAFF, BLYS, CD257, DTL, TALL1, THANK, TNFSF20, ZTNF4, TALL-1
host species Human
production host HEK293
tags N-hFc Tag
purity The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
endotoxin No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Lyophilized from sterile PBS, pH 7.4. Typically, 5 % - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize by gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

Flow cytometry analysis with 15 ug/mL Human BAFF Protein, hFc tag on Expi293 cells transfected with human BAFF-R (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human BAFF, hFc tagged protein binds to Human BCMA, mFc tagged protein in a linear range of 0.03-15.625 ng/ml.

ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human BAFF, hFc tagged protein binds to Human BAFF-R, mFc tagged protein in a linear range of 0.488-250.0 ng/ml.

ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human BAFF, hFc tagged protein binds to Anti-BAFF Neutralizing antibody in a linear range of 3.2-400 ng/ml.

Human BAFF Protein with hFc Tag on SDS-PAGE under reducing condition.


PRODUCT

  • SKU

    DMP2206-50ug

  • Size

    50ug

  • Price

    $648

1
  • SKU

    DMP2206-100ug

  • Size

    100ug

  • Price

    $971

1