Recombinant rabbit anti-human CD79B chimeric monoclonal antibody, clone 52A11

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PRODUCT

  • SKU

    DMR1108-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1108-500ug

  • Size

    500ug

  • Price

    $2,010

1
This recombinant rabbit monoclonal antibody is part of a collection of rabbit mAbs against druggable targets developed by direct cloning from immunized rabbit B cells without hybridoma fusion. All antibodies have been fully sequenced and extensively validated by FACS and ELISA assays. Typically, multiple clones against each target are available for customer testing. The clones are available for licensing once the druggability of the antibodies has been validated by the customer, saving tremendous development time and effort. Antibody gene libraries from antigen-positive B cells are also available for further screening if the listed antibodies do not meet the customer's needs.
target CD79B
alias AGM6, B29, IGB
gene id Uniprot ID: P40259
host species Rabbit
production host HEK293
reactivity Human
clonality Monoclonal
clone number 52A11
iso type Rabbit/Human Fc chimeric IgG1
conjugate Unconjugated
formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
applications Flow Cytometry, WB
dilution Flow Cyt 1:100; WB 1:1000
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 1 week at room temperature. Store at -20 °C to -80 °C for 12 months in lyophilized form. After reconstitution, store at 4 °C for 2-7 days or at below -20 °C for up to 3 months.

Images

Flow cytometry analysis with Anti-CD79B (52A11) mAb on Expi293 cells transfected with human CD79B (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Anti-CD79B antibody at 1/1000 dilution. Lanes: RAJI (human Burkitt's lymphoma B lymphocyte), whole cell lysate. Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution. Predicted band size: 26 kDa, observed band size: 24 kDa

Flow cytometry analysis of antigen binding of anti-human CD79B mAb. (A) The antibody does not bind to 293T cells that do not express CD79B. (B) A clear peak shift of The antibody was seen compared to the control when incubated with CD79B-expressing Raji cells, indicating strong binding of The antibody to CD79B. Antibodies were incubated at 5 ug/mL.

Related Products

PRODUCT

  • SKU

    DMR1108-100ug

  • Size

    100ug

  • Price

    $669

1
  • SKU

    DMR1108-500ug

  • Size

    500ug

  • Price

    $2,010

1

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